This is the simplest of all the surveys to analyze. To get a total
number of organisms observed at each site, sum up all the different
species of organisms observed along each transect, then add the
totals for each transect line.
Example :
Middle Transect
1
2
3
4
5
Total
Sea Urchins
Echinometra
mathei
‘Ina
Rock boring
urchin
0
2
8
5
1
16
Echinometra
oblonga
‘Ina
Black boring
urchin
1
5
4
9
3
22
Echinothrix
calamaris
Wana
Banded sea
urchin
0
0
0
1
0
1
Echinothrix
diadema
Wana
Black sea
urchin
0
0
1
0
1
2
Heterocentrotus
mammillatus
Peni
Slate pencil
urchin
0
1
0
0
0
1
Tripneustes
gratilla
Hāwa‘e
Collector
urchin
0
2
2
1
0
5
Site 1
Inner Transect
Middle Transect
Outer Transect
Total
Sea Urchins
Echinometra
mathei
‘Ina
Rock boring
urchin
5
16
20
41
Echinometra
oblonga
‘Ina
Black boring
urchin
8
22
29
59
Echinothrix
calamaris
Wana
Banded sea
urchin
0
1
3
4
Echinothrix
diadema
Wana
Black sea
urchin
0
2
5
7
Heterocentrotus
mammillatus
Peni
Slate pencil
urchin
0
1
4
5
Tripneustes
gratilla
Hāwa‘e
Collector
urchin
0
5
9
14
Substrate
The first step is to calculate percent cover of rock, sand and
coral. Start with % sand by adding up all the percentages of sand
for each quadrat. Divide that sum by the total number of quadrats
to get an average percent cover of sand for the entire site. Do
the same for % rock and % coral. You should be able to see the
dominant type of substrate.
Now that you have a clear picture of substrate for the site, you
need to look back at the percent cover of coral. You have calculated
the amount of coral present at the site, but have not yet calculated
the percentage of each type of coral. Do the same as you did for
percent rock, sand and coral. Add up the percentages for each type
of coral (one type at a time) and divide that sum by the total
number of quadrats to get an average percent cover of each type
of coral for the entire site. You should be able to see the most
abundant type of coral.
Example :
Outer Transect
Quad #
% Sand
% Rock
% Coral
Cauliflower
Lobe
Antler
Finger
Plate
1
80
20
0
0
0
0
0
0
2
70
25
5
10
60
10
10
10
3
50
40
10
30
60
0
0
10
4
60
10
30
15
50
10
10
15
5
50
25
25
10
50
10
20
10
Total
310
120
70
65
220
30
40
45
Average
62 %
24 %
14 %
13 %
46 %
8 %
8 %
5 %
Site 1
% Sand
% Rock
% Coral
Cauliflower
Lobe
Antler
Finger
Plate
Inner Transect
85
15
0
0
0
0
0
0
Middle Transect
75
17
8
60
25
15
0
0
Outer Transect
62
24
14
13
46
16
20
5
Total
222
56
22
73
71
31
20
5
Average
74 %
19 %
7 %
24 %
24 %
10 %
7 %
2 %
Water Quality
In determining water quality, it is a good idea to analyze multiple
samples from different areas within the site. This will take into
account varying conditions within the site. Most water quality
tests will give you a number result. For those tests, you can simply
take the average number of all the test samples. Other tests like
coliform bacteria may give you a positive or negative result. You
can either choose to take the most dominant result as your overall
result or you can use the locations of each test sample in your
data analysis to show how different areas within the site may be
influenced by other factors.
Example :
Site 1
Test
Sample 1
Sample 2
Sample 3
Total
Average
Nitrate (ppm)
20
20
5
45
15
Phosphate (ppm)
2
2
1
5
2
pH
6
7
6
19
6
Temperature ( ° C)
30
32
32
94
31
Fish Abundance & Diversity
As you did in the benthic organism survey, simply add up the total
number of tallies (in the count column) for each species of fish
and record that total in the sum column. Once you have summed up
the totals for all of the species of fish, rank them in order of
abundance. The species of fish with the most tallies would be ranked
number one. The species of fish with the second highest number
of tallies would be ranked number two and so forth. The tallies
and ranking show fish abundance. The number of different species
observed will show fish diversity.
Example :
Middle Transect
Rank
Count 1
Sum 1
Count 1
Sum 1
Total
Butterflyfish
Chaetodon auriga
Threadfin
2
llll
4
ll
2
6
Chaetodon quadrimaculatus
Lauhau
Four Spot
5
0
ll
2
2
Chaetodon lunula
Kīkākapu
Racoon
1
lllll
5
lll
3
8
Chaetodon miliaris
Lauwiliwili
Milletseed
3
lll
3
lll
2
5
Chaetodon ornatissimus
Kīkākapu
Ornate
4
ll
2
l
1
3
Chaetodon multicinctus
Multiband
4
0
lll
3
3
Site 1
Inner Transect
Middle Transect
Outer Transect
Total
Butterflyfish
Chaetodon auriga
Threadfin
3
6
4
13
Chaetodon quadrimaculatus
Lauhau
Four Spot
3
2
7
12
Chaetodon lunula
Kīkākapu
Racoon
7
8
5
20
Chaetodon miliaris
Lauwiliwili
Milletseed
2
5
8
15
Chaetodon ornatissimus
Kīkākapu
Ornate
5
3
7
15
Chaetodon multicinctus
Multiband
1
3
6
10
Drawing Conclusions
This is a difficult process to internalize for most students because
it requires them to use their critical thinking skills in a way
that they probably have little or no experience in doing. Students
drawing conclusions from data is like a detective solving a mystery
case from evidence. The overall questions in data analysis are: What
happened? and How come?
The students will need a lot of guidance and direction in drawing
these conclusions. Try going through one example with the students
then break them up into smaller groups to see what they come up
with. Once they get the hang of it, you'll be pleasantly surprised
at how well they can start to see and understand the big interconnecting
picture of ocean ecosystems. Reserve quite a bit of time for this
phase of the project.
Benthic Organisms
Guiding Questions : What organisms are most
abundant at the site and why?
To answer these questions you cannot just look at the organisms
themselves since organisms are influenced by so many different
factors. Habitat is arguably the most important factor here so
if you did not include water quality in your survey that's okay.
Ask yourself how the abundance and diversity of organisms would
be affected by factors such as :
Type and percent cover of substrate
Water temperature
Salinity
Tidal ebb and flow
Open ocean or enclosed site
Wave action
Water quality
Supply of food
Predators
Example :
RESULTS
(What
happened?)
CONCLUSION
(How come?)
Rock
boring urchins were the most abundant.
Rock
was the dominant substrate. Rock is the habitat of
rock boring urchins.
Rock boring
urchins are protected from high wave action by
boring into rock.
Rock boring
urchins are protected from predators by boring into
rock.
Christmas
tree worms were the least abundant.
Lobe
coral was the least abundant of of all the corals. Lobe
coral is the primary habitat of Christmas tree worms.
Site
1 had a low diversity of organisms.
Site
1 has a lot of wave action that makes it difficult
for organisms to survive.
Site 1
is an enclosed area that is half drained at low
tide. Not too many organisms can survive that kind of intertidal
habitat.
Substrate
Guiding Questions : What type of substrate
is the most abundant at the site and why?
What type of coral the most abundant and why?
Unlike benthic organisms, substrate is not affected by too many
factors. Ask yourself substrate would be affected by factors such
as :
Water depth and salinity (coral)
Wave action
Open ocean or enclosed site
Water quality
Example :
RESULTS
(What happened?)
CONCLUSION
(How come?)
The
percent cover of sand and rock were about the same.
Site
1 is a deep open ocean area, favorable conditions for both
sand and rock.
Lace
coral was the least abundant type of coral.
Lace
coral is one of the most delicate types of coral so they
are rarely found in areas of high wave action like site
1.
Water Quality
Guiding Questions: What is the overall water
quality?
The results of the different tests will be linked to environmental
factors. Some of the parameters such as nitrates, phosphates and
coliform bacteria are inter-related. Ask yourself how overall water
quality would be affected by factors such as :
Type and percent cover of substrate
Tidal ebb and flow
Open ocean or enclosed site
Wave action
Run-off
Example :
RESULTS
(What happened?)
CONCLUSION
(How come?)
Site 1 had a
high level of turbidity.
Site
1 is a completely enclosed area with no connection
to the ocean.
Water is unable to get mixed around.
Site
1 had a low level of dissolved oxygen.
Site
1 is a completely enclosed area with no connection
to the ocean. Water is unable to get mixed around.
There
is very little wave action that helps to add oxygen
into the area.
Fish Abundance & Diversity
Guiding Questions : How abundant and diverse
is the fish population and why?
Much like benthic organisms, fish
populations are affected by a number of factors. Most of our coastal
fish are reef fish, so naturally the status of reefs would be a
large influence. Fishing also comes into play here more than since
many of our reef fish are caught for either human consumption or
human entertainment. Ask yourself how the abundance and diversity
of fish would be affected by factors such as:
Type and percent cover of substrate
Tidal ebb and flow
Open ocean or enclosed site
Wave action
Water quality
Supply of food
Predators
Example :
RESULTS
(What
happened?)
CONCLUSION
(How come?)
Fish
abundance is low.
The
coral reef at site 1 is healthy but small in area. It doesn't
provide much space or food for a large number of fish.
Fish
diversity is high.
The
coral reef at site 2 is extensive and healthy. The reef
provides habitat and food for a wide variety of fish.